The squad is made up of 28 forwards and 18 backline players and sees Paul de Villiers (flanker), Riley Norton (lock/loose forward), Carlu Sadie (prop) and Ruben van Heerden (lock) called up for the first time among the forwards, while Vusi Moyo (flyhalf) and Jaco Williams (wing) are new backline inclusions.
Several of the players featured for the Springboks in their 80-31 win over the Barbarians, a game that did not was not a capped international.
There is a return to the squad for scrumhalves Herschel Jantjies, who was a member of the 2019 World Cup-winning squad but who has not played for the Springboks in almost three years, and Embrose Papier, who played the last of his seven tests in 2018.
The match against England will be followed by fixtures against Scotland in Pretoria on July 11 and then Wales in Durban on July 18.
The same squad will also be used for a one-off test against Argentina in Buenos Aires on August 8.
Squad:
Backs: Kurt-Lee Arendse (Bulls), Damian de Allende (Wild Knights), Andre Esterhuizen, Aphelele Fassi (both Sharks), Quan Horn (Lions), Herschel Jantjies (Bayonne), Cheslin Kolbe (Tokyo Sungoliath), Jesse Kriel (Canon Eagles), Manie Libbok (Kintetsu Liners), Canan Moodie (Bulls), Vusi Moyo (Sharks), Embrose Papier, Handre Pollard (both Bulls), Cobus Reinach (Stormers), Edwill van der Merwe (Sharks), Damian Willemse (Stormers), Grant Williams, Jaco Williams (both Sharks)
Forwards: Paul de Villiers (Stormers), Lood de Jager (Wild Knights), Ben-Jason Dixon (Stormers), Thomas du Toit (Bath), Pieter-Steph du Toit (Toyota Verblitz), Eben Etzebeth (Sharks), Johan Grobbelaar, Cameron Hanekom (both Bulls), Siya Kolisi (Sharks, captain), Wilco Louw (Bulls), Malcolm Marx (Kubota Spears), Ntuthuko Mchunu (Stormers), Franco Mostert (Honda Heat), Ox Nche (Sharks), Ruan Nortje (Bulls), Riley Norton, Zachary Porthen, Evan Roos (all Stormers), Carlu Sadie (Bordeaux Begles), Gerhard Steenekamp (Bulls), Vincent Tshituka (Sharks), Ruben van Heerden (Stormers), Marco van Staden (Bulls), Andre-Hugo Venter (Stormers), Boan Venter (Edinburgh), Jan-Hendrik Wessels, Cobus Wiese (both Bulls), Jasper Wiese (Urayasu D-Rocks).