The round will get under way on Wednesday, February 4, as the defending champions travel to Cape Town Stadium to face Stellenbosch FC.
Chiefs ended their 10-year trophy drought by claiming the Nedbank Cup title last season and have carried that good form into this campaign. But they face a tough Last 32 tie against coach Gavin Hunt’s Stellenbosch.
That same evening, giants Mamelodi Sundowns host second-tier Gomora United at Loftus Stadium, defending a proud record of not having lost to lower-league opponents in 23 previous Nedbank Cup games since the tournament was first played in its current guise in 2008.
They have recorded 22 wins and a single draw against University of Pretoria, where they advanced on penalties.
There will be two games on Friday, February 6, as Durban City host Chippa United in an all-Betway Premiership clash, before a meeting between two third-tier sides as CR Vasco da Gama entertain fellow ABC Motsepe League outfit Luthuli Brigades at Athlone Stadium in Cape Town.
There are six games on Saturday, February 7, with the pick of those a clash between third-tier TTM and 10-time champions Orlando Pirates at Moses Mabhida Stadium in Durban.
That is a long way from TTM’s Limpopo home, but gives them a chance to cash in on the tie at the 2010 World Cup venue, where a sizeable crowd will be expected.
Little-known ABC Motsepe League sides FC Cardinals and Jacksa Spears clash at Goble Park in Bethlehem, while third-tier Army Rockets host Motsepe Foundation Championship leaders Casric Stars at Olympia Park in Rustenburg.
There are four more games on Sunday, February 8, including two all-Premiership fixtures as TS Galaxy welcome Marumo Gallants to Solomon Mahlangu Stadium, and Siwelele host Richards Bay in Bloemfontein.
The final two matches in the round will be played on Tuesday, as third-tier Super Soweto United M17 host Milford FC at Dobsonville Stadium, and AmaZulu travel to Old Peter Mokaba Stadium to face Polokwane City.
2026 Nedbank Cup Last 32 fixtures:
Wednesday, February 4
19h00: Stellenbosch FC vs Kaizer Chiefs - Cape Town Stadium
19h00: Mamelodi Sundowns vs Gomora United - Loftus Versfeld Stadium
Friday, February 6
19h00: Durban City vs Chippa United - Chatsworth Stadium
19h00: CR Vasco da Gama vs Luthuli Brigades FC - Athlone Stadium
Saturday, February 7
15h00: Upington City vs Magesi FC - Mxolisi Dicky Jacobs Stadium
15h00: Tshakhuma vs Orlando Pirates - Moses Mabhida Stadium
15h00: Highbury FC vs Sekhukhune United - Nelson Mandela Bay Stadium
15h00: FC Cardinals vs Jacksa Spears - Goble Park Stadium
15h00: Army Rockets vs Casric Stars - Olympia Park Stadium
18h00: Lamontville Golden Arrows vs Orbit College - King Zwelithini Stadium
Sunday, February 8
15h00: TS Galaxy vs Marumo Gallants - Solomon Mahlangu Stadium
15h00: Hungry Lions vs University of Pretoria - Mxolisi Dicky Jacobs Stadium
15h00: Mkhambathi FC vs Lerumo Lions - Harry Gwala Stadium
18h00: Siwelele vs Richards Bay - Dr Molemela Stadium
Tuesday, February 10
19h00: SSU M17 FC vs Milford FC - Dobsonville Stadium
19h00: Polokwane City vs AmaZulu FC - Old Peter Mokaba Stadium